Wisconsin Discovery Portal

Researcher: Ronald Raines

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Areas of Expertise
  • Cellular regulation
  • Molecular genetics
  • Protein structure
  • Hormone action
  • Cell biology
  • Chemical biology, protein chemistry and engineering, enzymology, biofuels
  • Chemical basis and biological purpose for protein structure and protein function
  • How biological phenomena can be explained with the principles of chemistry
  • Relationship between amino-acid sequence and protein function (or dysfunction)
  • Creation of novel molecules with desirable properties
  • Stability of collagen and other proteins
  • Processes to synthesize proteins and convert crude biomass into useful fuels and chemicals
  • Nucleases, including ribonuclease A
  • An RNA-cleaving enzyme that is in a human clinical trial as an anti-cancer agent
Web Site Ronald Raines' University Web Page
Curriculum Vitae (CV)
Issued Patent(s)
  • 9,085,590 – Protecting groups for boronic acids, granted July 2015.
  • 8,962,001 – Nuclease inhibitors and methods for their use, granted February 2015.
  • 8,871,916 – Diaryl phosphine compounds, granted October 2014.
  • 8,834,918 – Modified multilayered film, granted September 2014.
  • 8,722,878 – Biomass hydrolysis, granted May 2014.
USPTO Published Applications
  • 20140335593 – Cytotoxic ribonuclease variants, November 2014.
  • 20140235851 – Chemical transformation of lignocellulosic biomass into fuels and chemicals, August 2014.
  • 20140220651 – Biomass hydrolysis, April 2014.
  • 20140038288 – Cytotoxic ribonuclease variants, February 2014.
  • 20130344563 – Nuclease inhibitors and methods for their use, December 2013.
Recent Publication(s)
  • Convenient synthesis of collagen-related tripeptides for segment condensation. Ellison AJ, VanVeller B, Raines RT. Biopolymers. 2015 Jul 14. doi: 10.1002/bip.22700. [Epub ahead of print]
  • Selective inhibition of prolyl 4-hydroxylases by bipyridinedicarboxylates. Vasta JD, Raines RT. Bioorg Med Chem. 2015 Jul 1;23(13):3081-90. doi: 10.1016/j.bmc.2015.05.003. Epub 2015 May 11.
  • Thioamides in the collagen triple helix. Newberry RW, VanVeller B, Raines RT. Chem Commun (Camb). 2015 May 28;51(47):9624-7. doi: 10.1039/c5cc02685g.
  • Optimized diazo scaffold for protein esterification. Mix KA, Raines RT. Org Lett. 2015 May 15;17(10):2358-61. doi: 10.1021/acs.orglett.5b00840. Epub 2015 May 4.

View Ronald Raines' publications at Google Scholar.
Recent Artistic Works
  • Hyrax Energy Inc.
  • Memorial Sloan Kettering Cancer Center, Organic Synthesis Core Facility
  • Rice University, Chemistry, Biosciences
Research Tools
  • Isothermal Titration Calorimeter
  • Differential Scanning Calorimeter
  • Analytical Ultracentrifuge
  • Circular Dichroism Spectrometer
  • Fourier Transform Infrared Spectrometer
  • Surface Plasmon Resonance Detector
Research Facilities
  • Biophysics Instrumentation Facility
E-mail Address [email protected]
Phone Number 608-262-8588
Current University UW–Madison
Department Biochemistry/Chemistry
Title Professor
Other Appointments Director, Biophysics Instrumentation Facility
Address Line 1 371 DeLuca Biochemistry Laboratories
Address Line 2 433 Babcock Drive
City Madison
State WI
Zip Code 53706-1544
Bachelor's Degree Sc.B., Massachusetts Institute of Technology, Biology and Chemistry, 1980
Master's Degree A.M., Harvard University, Chemistry, 1982
PhD Ph.D., Harvard University, Chemistry, 1986
Other Degrees
Technologies Available for Licensing Hyperstable Collagen Mimics

Fluorescent Assays with Improved Sensitivity

Methods and Reagents for Appending Functional Groups to Proteins

Cytotoxic Ribonuclease Variants

Chemical Synthesis of Reagents for Peptide Coupling

Nuclease Inhibitors and Methods for Their Use

Cytotoxic Ribonuclease Variants

Cell-Permeable Green Fluorescent Protein

Synthesis of Magnetic Resonance Imaging Contrast Agents Using Ring-Opening Metathesis Polymerization

Stable Collagen Mimics

Stable, Oxidation-Resistant Inhibitors for Reducing Ribonuclease Contamination

Staudinger Ligation Method for Rapid and Reliable Chemical Synthesis of Proteins

Multilayered Film for Delivering Proteins and Other Small Molecules into Cells

Strong, Stable, Semisynthetic Collagen Mimic for Wound Healing, Artificial Skin, Sutures and Leather

Efficient, Lower Cost Chemical Transformation of Lignocellulosic Biomass into Fuels and Chemicals

Protecting Groups for Boronic Acids

Bioreversible Protein Esterification

Traceless Staudinger Ligation for the Synthesis of Peptides and Proteins in Water

Simple, Gentle and Chemoselective Method of Synthesizing Diazo Compounds

New Disulfide-Bond Reducing Agent

High-Yielding Method for Converting Biomass to Fermentable Sugars for Biofuel Production

Improving Drug Delivery with Boronic Acids

Mild, Nontoxic Production of Fuels and Chemicals from Biomass

Improved Disulfide-Bond Reducing Agents