Wisconsin Discovery Portal

Researcher: Jon Wolff

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Areas of Expertise
  • Developmental pediatrics
  • Molecular genetics
  • Clinical genetics
  • Gene therapy approaches for neuromuscular and metabolic diseases
  • Ability to directly deliver plasmid DNA into muscle, liver, and other tissues
  • How plasmid DNA traverses cell membranes and enters nuclei
  • Mechanism by which plasmid DNA enters the cell and reaches the nucleus following transfection and ways to increase the number of transfected target cells
Web Site
Curriculum Vitae (CV)
Issued Patent(s)
  • 9,290,779 - Transfection compositions using amphipathic compounds, issued March 2016.
  • 9,217,145 - T7 phage peptide display system and uses thereof , issued December 2015.
  • 9,107,957 - Peptide-based in vivo sirna delivery system, issued August 2015.
  • 8,802,773 - In vivo polynucleotide delivery conjugates having enzyme sensitive linkages, issued August 2014.
  • 8,658,211 - Polyconjugates for in vivo delivery of polynucleotides, issued February 2014.
USPTO Published Applications
  • 20150273081 - Reversibly masked polymers, published October 2015.
  • 20120230938 - Polyconjugates for in vivo delivery of polynucleotides, published September 2012.
  • 20120172412 - In vivo polynucleotide delivery conjugates having enzyme sensitive linkages, published July 2012.
  • 20120165393 - Peptide-based in vivo sirna delivery system, published June 2012.
  • 20100004180 - Agents for the detection and modulation of b-cells and antibodies, published January 2010.
Recent Publication(s)
  • Protease-triggered sirna delivery vehicles. Rozema DB, Blokhin AV, Wakefield DH, Benson JD, Carlson JC, Klein JJ, Almeida LJ, Nicholas AL, Hamilton HL, Chu Q, Hegge JO, Wong SC, Trubetskoy VS, Hagen CM, Kitas E, Wolff JA, Lewis DL. J Control Release. 2015 Jul 10;209:57-66. doi: 10.1016/j.jconrel.2015.04.012. Epub 2015 Apr 14.

  • Dose response in rodents and nonhuman primates after hydrodynamic limb vein delivery of naked plasmid dna. Wooddell CI, Hegge JO, Zhang G, Sebestyén MG, Noble M, Griffin JB, Pfannes LV, Herweijer H, Hagstrom JE, Braun S, Huss T, Wolff JA. Hum Gene Ther. 2011 Jul;22(7):889-903. doi: 10.1089/hum.2010.160. Epub 2011 Apr 18.

  • Muscle damage after delivery of naked plasmid dna into skeletal muscles is batch dependent. Wooddell CI, Subbotin VM, Sebestyén MG, Griffin JB, Zhang G, Schleef M, Braun S, Huss T, Wolff JA. Hum Gene Ther. 2011 Feb;22(2):225-35. doi: 10.1089/hum.2010.113. Epub 2011 Feb 2.

  • Hybrid pet/ct for noninvasive pharmacokinetic evaluation of dynamic polyconjugates, a synthetic sirna delivery system. Mudd SR, Trubetskoy VS, Blokhin AV, Weichert JP, Wolff JA. Bioconjug Chem. 2010 Jul 21;21(7):1183-9. doi: 10.1021/bc900558z.
Recent Artistic Works
  • Roche Madison, Chris Wooddell
  • CHUL Research Center, Quebec City, Neurosciences
  • University of North Carolina at Chapel Hill, Gene Therapy Center
Research Tools
Research Facilities
E-mail Address [email protected]
Phone Number 608-263-5993
Current University UW–Madison
Department Volunteer Staff
Title Clinical Adjunct Professor
Other Appointments
Address Line 1 Box 9345 Waisman Center
Address Line 2 1500 Highland Avenue
City Madison
State WI
Zip Code 53705
Bachelor's Degree
Master's Degree
Other Degrees M.D., Johns Hopkins University, 1980
Technologies Available for Licensing Improved Phage Display System Enables Screening of Liver-Specific Peptides