Wisconsin Discovery Portal

Researcher: Richard Burgess

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Areas of Expertise
  • RNA polymerase and the regulation of transcription
  • Prokaryotic and eukaryotic RNA polymerases
  • Transcription machinery and its role in regulation of RNA synthesis
  • Structure and function of the initiation factor, E. coli RNA polymerase sigma-70 subunit, by a concerted use of protein and physical chemistry, monoclonal antibodies, molecular genetics, and biochemistry
  • Protein refolding
  • Gentle immunoaffinity chromatography
  • New technology to detect and isolate weak protein-protein interactions
Web Site Richard Burgess' Department of McArdle Laboratory for Cancer Research Website
Curriculum Vitae (CV)
Issued Patent(s)
  • 8,993,243 - Method for isolating weakly interacting molecules from a fluidic sample, issued March 2015.
  • 8,728,410 - Device for and method of extracting a fraction from a biological sample, issued May 2014.
  • 7,713,736 - Cell mimic platform and method, issued May 2010.
  • 7,241,580 - Immunoaffinity chromatography using epitope tags to polyol-responsive monoclonal antibodies, issued July 2007.
  • 5,981,835 - Transgenic plants as an alternative source of lignocellulosic-degrading enzymes, issued November 1999.
USPTO Published Applications
  • 20120178096 - Method for isolating weakly interacting molecules from a fluidic sample, published July 2012.
  • 20110212509 - Device for and method of extracting a fraction from a biological sample, published September 2011.
  • 20060110722 - Cell mimic platform and method, published May 2006.
  • 20050272919 - Immunoaffinity chromatography using epitope tags to polyol-responsive monoclonal antibodies, published December 2005.
  • 20030104423 - Sigma binding region of rna polymerase and uses thereof, published June 2003.
Recent Publication(s)
  • A brief review of common grammatical and scientific errors seen in reviewing protein purification manuscripts for 25 years. Burgess RR. Protein Expr Purif. 2016 Apr;120:106-9. doi: 10.1016/j.pep.2015.12.002. Epub 2015 Dec 10. Review.

  • Weak protein-protein interactions revealed by immiscible filtration assisted by surface tension. Berry SM, Chin EN, Jackson SS, Strotman LN, Goel M, Thompson NE, Alexander CM, Miyamoto S,┬áBurgess RR, Beebe DJ. Anal Biochem. 2014 Feb 15;447:133-40. doi: 10.1016/j.ab.2013.10.038. Epub 2013 Nov 9.

  • Key features of σs required for specific recognition by Crl, a transcription factor promoting assembly of RNA polymerase holoenzyme. Banta AB, Chumanov RS, Yuan AH, Lin H, Campbell EA,┬áBurgess RR, Gourse RL. Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):15955-60. doi: 10.1073/pnas.1311642110. Epub 2013 Sep 16.

  • Production and characterization of monoclonal antibodies to estrogen-related receptor alpha (ERRα) and use in immunoaffinity chromatography. Esch AM, Thompson NE, Lamberski JA, Mertz JE,┬áBurgess RR. Protein Expr Purif. 2012 Jul;84(1):47-58. doi: 10.1016/j.pep.2012.04.020. Epub 2012 May 4.


View Richard Burgess' publications at PubMed.
Recent Artistic Works
Collaboration
  • Intra-University Collaboration: Cellular and Molecular Biology, Biomedical Engineering, Bacteriology
  • Indian Institute of Technology Guwahati, Biotechnology
Research Tools
Research Facilities
E-mail Address [email protected]
Phone Number 608-263-2635
Current University UW–Madison
Department Oncology / Biomedical Engineering
Title Emeritus Professor
Other Appointments Honorary Associate / Fellow
Address Line 1 7455 Wisconsin Institutes for Medical Research
Address Line 2 1111 Highland Avenue
City Madison
State WI
Zip Code 53706
Bachelor's Degree B.S., California Institute of Technology, Chemistry, 1964
Master's Degree
PhD Ph.D., Harvard University, Biochemistry and Molecular Biology, 1969
Other Degrees
Technologies Available for Licensing Mouse Monoclonal Antibodies to the Human Nuclear Receptor, Estrogen-Related Receptor Alpha

Monoclonal Antibody to Eukaryotic RNA Polymerase II

Mouse Monoclonal Antibody Against the Sigma70 Subunit of E. coli RNA Polymerase

Mouse Monoclonal Antibody Against the Alpha Subunit of E. coli RNA Polymerase

Microfluidics Platform and Method That Mimic the Cellular Environment

An Epitope Tag for Gentle Immunoaffinity Chromatography

Mouse Monoclonal Antibodies to Epstein-Barr Virus Nuclear Antigen 1 (EBNA1)

Device for Efficiently Extracting a Fraction Containing Nucleic Acids or Other Desired Material from a Biological Sample

Method for Isolating Weakly Interacting Molecules from a Fluidic Sample